22 June 2023 1 8K Report

Am a student working on '' INVESTIGATING THE EFFECT OF TESTOSTERONE ON GLUCOSE REGULATION IN THE LIVER. Am working with HepG2 cells. Am going to evaluate the effect of testosterone on glycogen synthesis and glucose uptake.

Thanks

More Patrick Asi's questions See All
How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

Can we eliminate the stress singularity at the tip of the crack by manipulating the elastic constants?

The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.

25 July 2024 9,976 3 View

How to account for semi-opaque obstruction in visual field "anchored" to bottom?

I have seen two kinds of explanations given for the phenomenon that I try to draw in the attached png document. One explanation says that an air bubble creates the experience of a solid black...

16 June 2024 6,493 1 View

Can cross-slip, twinning and fracture systems, under applied loadings, receive the same mathematical theory?

Cross-slip, twinning and fracture are major deformation modes adopted by loaded materials. It appears sound that these apparently different deformation mechanisms can be analysed on the equal manner!

15 June 2024 8,477 3 View

Which term is preferable between Fictional Language, Fictitious Language, Artificial Language, or Constructed Language in fiction?

The terms Fictional Language, Fictitious Language, Artificial Language, or Constructed Language in fiction has been used interchangeably in papers that I have read. Are there differences in these...

05 June 2024 3,453 7 View

Which Hybrid Sorghum Varieties Are Most Effective in Saudi Arabia for Silage?

Investigating use of Sorghum as part of Regenerative Agriculture management system using cover crops & mower-crimping, Alley Cropping, sheep manure in a zero till management system with...

18 April 2024 2,389 3 View

Can cases in multiple case study be bounded by gender?

I'm conducting a multiple case study, and I'm interested in exploring a phenomenon from the perspectives of male and female participants.

14 April 2024 4,352 1 View

Hello everyone, please can somebody assist me here.?

I need to prepare Na2HPO4/Citrate buffer pH 5.0, it is possible to prepare 1M, 0r 0.5M if yes, please how? Thank you I want to crystallize my protein with this buffer? i need 0.1M working...

03 April 2024 10,006 0 View

Please is the conference organized by World Academics legitimate?

The following are the details of the conference: Name of event: International Conference on Community Health Nursing and Public Health (ICCHNPH) Venue: Brno, Czech Republic Date: 9th -10th...

25 March 2024 996 1 View

Regarding Sample Treatment in cell culture ?

For cell culture , if want to study about the protein or gene expression ? The treatment need to dilute with medium without fbs & pen strep or complete medium. What happen , if the treatment...

19 March 2024 399 2 View

Similar questions and discussions
How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close