Why does beta-carotene concentration increase during the autumnal exposure in lichens samples?

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Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Use magnetic bead purification after gel electrophoresis?

Hi, I am working with Invitrogen Seamless cloning which requires 200 ng/ul insert DNA fragment. Protocol recommends to use restriction enzyme to digest pre-cloned plasmid and then elute specific...

25 June 2024 971 2 View

How to set up focus groups in the face of generational barriers?

I am carrying out a research project in which I am conducting focus groups with older men and women to study the musical stereotypes learned in traditional Spanish oral music groups, but I am...

10 May 2024 3,852 3 View

What is the best LC/MS system for surfactant analysis?

We are an R&D laboratory focused on anionic, cationic, and zwitterionic surfactants and usually tackle surfactant synthesis and analysis of products from customers. In particular, we deal with...

29 April 2024 8,927 1 View

Why is there no 28S RNA in mouse sceletal muscle?

We are isolating total RNA from mouse sceletal muscle, using the beads beater with the sample already being in Trizol, afterwards chloroform extraction and finally Qiagen RNeasy kit. On the TAPE...

29 April 2024 3,952 5 View

What’s the most used inclusive leadership assessment scale?

I’m looking for an inclusive leadership scale to include in a mediation analysis with psych safety and workplace belonging, what would you use

27 April 2024 6,072 2 View

COMET ASSAY: Why do cells disappear?

Greetings, I am trying to set up an alkaline Comet Assay. I have no previous experience with this type of assay, unfortunately, so I am doing a lot of trial and error. After embedding my cells in...

23 April 2024 6,534 0 View

In what ways does the Sysmex DI-60 streamline laboratory workflow and improve laboratory efficiency in terms of reduced TAT?

This question is directed on the performance of the Sysmex DI-60 digital morphology analyzer and its contributions on the improvement in the laboratory workflow. I am curious as to what...

10 April 2024 3,069 1 View

Aside from a lack of understanding, what other variables contribute to antibiotic diversion in low-income countries such as the Philippines?

It is known that self-medication with antibiotics is an arising problem, becoming a major constituent to antibiotic resistance. Moreover, self-medication heightens the possibility of developing...

09 April 2024 6,644 1 View

What are the most prevalent antibiotic resistance patterns among outpatient populations in the Philippines?

Antibiotic resistance is a pressing global and local health concern. Outpatient settings play a crucial role in antibiotic consumption and the emergence of resistant bacteria due to the frequent...

09 April 2024 6,004 0 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

What are the modern approaches to the organization and sustainable management of greenery in urbanized cities?

What are the modern approaches to the organization and sustainable management of greenery in urbanized cities? How can ecological and recreational functions be introduced into the landscape and...

23 July 2024 7,292 4 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

Is it possible to estimate population using animal footprint?

I'm trying to use cassowary footprint to estimate their population since their footprints are distinguishable from other animal on the field. is it really possible to do that? since it's really...

18 July 2024 3,729 7 View

¿Where to buy the Aspergillus oryzae NSAR1 strain?

Greetings: I am looking to buy/obtain the auxotrophic strain Aspergillus oryzae NSAR1, but I just have found one culture collection from Japan but haven't received any answer. Could you provide...

11 July 2024 6,847 0 View

I am carrying out an experiment on Trichoderma. How can I measure OD of the fungal growth after 7 days?

I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....

11 July 2024 6,251 1 View

Using Kruskal-Wallis-Test when analyzing abundance and diversity indices?

I'm analyzing abundances from various ecological surveys. So data was collected across multiple independent years from three sites within each year. The years fall under two temperature...

26 June 2024 2,798 2 View

What are these aggregates in activated sludge flocs?

I've been working with activated sludge samples and have encountered a challenge in identifying certain structures/organisms that appear abundantly within the flocs during optical microscopy...

23 June 2024 9,584 1 View

How can lichens and water creatures indicate levels of pollution and why do we use lichens as indicators of pollution levels?

17 June 2024 1,656 4 View

Please tell me the scientific guidelines (books) on Trombidiformes ticks?

books and scientific papers on the morphology, taxonomy and so on of ticks of the suborder Trombidiformes

17 June 2024 948 2 View