This is a photo of P. aeruginosa grown on a medium with the L-lysine-α-oxidase enzyme (electron microscopy). Has anyone observed anything? Why do you think the cells look wrinkled? What adaptive process underlies this?
Are you sure these cells are abnormal? Did you also images of the same strain grown without antibiotic?
Also, what antibiotic is this?
This is a P. aeruginosa cultivated on a normal medium. I see a very smooth surface compared to the previous photo. The first photo was of a P. aeruginosa that was incubated with the enzyme L-lysine-α-oxidase. I'm sorry, I was wrong, the bacterium grew with an enzyme, not with an antibiotic.
Can a cell wall look like this under the influence of an enzyme?
Is the enzyme added to the medium so outside the cells? Or expressed so cytoplasmic?
Then I am a bit perplexed. I could imagine the enzyme might have an effect on the peptidoglycan but its it's hard to imagine how it would cross the OM to act.
You might want to do some controls to be sure it is the action of the enzyme directly on the cells or something to do with the endproduct of the reaction.
Do the cells stop growing or slow down so that you have a phenotype to observe?
We assume that this is the initial stage of the destruction of the walls of bacteria. Visually, the growth on the medium with the enzyme was more meager than on the usual medium.
On these two photos lengths of bacteria is quite different. Also magnification is .different. There is a possibility (even if not a great one) that some beam damage did occur at higher magnification.
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