How to detect spurious correlation in a statistical dataset with binary variables?

17 January 2021 7,892 25 View

Can somebody explain to me why RNA looks like this on RNA electrophoresis picture down below?

I extract the RNA from muscle tissue using Qiagen RNeasy Micro kit (50) (kat.74004). Everything fine with RNA concentration - about 300-500 ng/microliter. Before putting it into wells I mix my RNA...

19 November 2020 2,449 5 View

Can you help me figure out how many EDTA I need to add in the solution?

Composition 2X Loading dye for RNA electrophoresis 95% formamide 0.025% SDS 0.025% bromophenol blue 0.025% xylene cyanol FF 0.025% ethidium bromide 0.5 mM EDTA. I wanna to prepare 10 ml of the...

10 November 2020 4,759 3 View

RNN/LSTM Networks : How to take into account different values of times on different time-series?

LSTM tools of the Matlab allow us to perform classification of the time-series. It is assumed, that different time-series can have different length, but they should have same interval of...

13 October 2020 3,484 3 View

Deep Learning : How on one model to take into account both dynamic (time-series) and static features ?

Matlab tools for Deeep Learning allow us to solve classification tasks with static features (e.g., pixels values of images) and tasks with dynamic features (time-series). For time-series it is...

06 September 2020 9,942 6 View

How to apply Operational and Dynamic Routines to Organization Design?

Dear colleagues, I'm working on methodology of transforming existing organization for manufacturing companies. Could you please guide me on how to approach the following questions: 1. How to...

08 July 2020 1,754 7 View

How to perform the ordinary one-way ANOVA analysis to compare the pharmacological effects for different drug-like substances?

The one-way ANOVA analysis is important to find out whether measured pharmacological effects (the means and medians) observed for three or more drugs are distinct or they are due to a chance....

11 May 2020 4,605 6 View

How to evaluate two-dimensional probability based on two one-dimensional (marginal)?

23 April 2020 6,418 9 View

Does anybody have experimental data on ferroresonance phenomena?

Does anybody have a reliable experimental data on ferroresonance phenomena in a transformer (single- or three-phase)? Thanking you in anticipation, Sergey Zirka

17 April 2020 8,656 4 View

Are there any changes in the tensional behavior of single-wall carbon nanotubes (CNT) with different chiral indexes?

We simulated (LAMMPS program) the tensional behavior of CNTs with different chiral indexes (10, 10; 0, 10, and 10,0) using the original CH.airebo potentials (Stuart et al., 2000). The plot...

16 January 2020 8,147 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View