Why do we use PEI (polyethylenimine) in protein solubility? and what about Sarcosyl?

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Protein solubilization

could someone please help me to know how to calculate the molarity of Ethanol. For in stance, if i want 6M ethanol, how much do need from 100% concentration?

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Should I use S-tag or His-tag?

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

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Why might my GFP tag be dissociating from my plasmid after transfection?

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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What is the most suitable server for predicting protein structures in bacteria?

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Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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Are protein samples in SDS compatible with ELISA?

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How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

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What's the concentration recommend for recombinant protein use in western blotting?

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Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

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What urea buffer can i use for biotagged protein extraction from streptavidin beads?

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Sanath Kumar Janaka

PEI is used to precipitate the DNA in the solution.

Deepti Agrawal

PEI is a cationic polymer which allows a protein to be more efficiently cationized and maintains protein function as much as possible by limited chemical conjugation. It is therefore used as attachment promoter and for efficient protein Transduction into living cells . It is also used for transfection as quoted by Arjan.

David Nyakundi

Thank you very much friends for taking time to answer my question. God bless you all.