On the mycotoxin determination methods for what purpose we use internal standards? Does it have any advantages that using external standard does not have? Why do we just not use external standards?
The principal advantage of using an internal standard in any method is that - if chosen properly - you are quite sure, that the recovery of the analyte is identical to the standard.
Using an external standard a similar recovery to the analyte has always to be proven.
Thus in a quantitative method, there are large advantages in using an internal standard.
You need an internal standard to:
1- compared with your analyte.
2- validate your methodology and use then as a reference method.
The external standard is used to build the calibration curve to quantify then your analyte in the chromatograph.
Internal standard is primarily used to increase the accuracy and precision of analytical methods that have large inherent variability.
Carbon13-labelled mycotoxins have been become quite popular for LC-MS/MS based (multi-)mycotoxin analysis in the recent years as they compensate matrix effects related to electroyspray ionization process
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