To elute your isolated RNA, pre-heated RNA isolation buffer is added to ensure proper RNA recovery. By adding elution buffer, magnetic beads and sample forms a homogenous solution during elution.
Hello Koroma,
This is a greater concern for RNA extraction. RNA degradation often occurs due to improper sample storage or inefficient lysis, assuming of course samples are eluted with RNase-free water. During RNA extraction, 10 mM Tris at pH 8-9 is typically used. DNA is more stable at a slightly basic pH and will dissolve faster in a buffer than water. RNA, can tolerate a slightly acidic pH and dissolves readily in water.For maximal RNA elution, allow the buffer to stand in the membrane for a few minutes before centrifugation. For applications requiring intact RNA, elution buffer is the best choice.
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