Hello everyone,
I have Top10 cells transformed with an ampicillin-resistant plasmid. I find that these cells can grow in LB medium but not on LB Agar plates with 50 ug/mL ampicillin. Do you know the reason for this phenomenon?
Thank you!
Are you starting from a glycerol stock?
Yes
When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...
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Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...
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Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...
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Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
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Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
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Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
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The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...
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Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?
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