Emma Thompson

3 Questions 17 Answers 0 Followers

Questions related from Emma Thompson

Why isn't carbenicillin/ampicillin inhibiting growth of a susceptible E. coli strain (TOP10)?

I'm doing disc diffusion experiments with E. coli TOP10 carrying my plasmid. I noticed that there was no difference between the TOP10 carrying the empty vector (my control) and TOP10 carrying my...

19 May 2023 5,887 5 View

Why can't I get confluent growth/a bacterial lawn of recombinant E. coli TOP10 (details below)?

As a pre-requisite for doing an antimciroial susceptibility disc diffusion method, I have been trying to determine at which OD600nm there are X CFU/mL as per the standardised Kirby Bauer disc...

02 May 2023 5,110 9 View

Why is my commercially synthesised plasmid 2kbp smaller after miniprep from E coli TOP10?

I have my gene synthesised and cloned into pGenDONR by Genscript = 6584 bp. I transformed E coli TOP10 and miniprepped the plasmid for propagation and storage. On an agarose gel, the...

24 November 2021 2,798 8 View