Generally, the 0.25M sucrose solution is the most common organic solution which is used for tissue homogenate preparation. There are many specific buffer solutions which are used for tissue homogenate preparation for specific enzyme assay. Please provide some references about the use of 0.25M sucrose solution and its advantages as well as disadvantages associated with its use over other buffers. Thank you.
Krishna Pada Singha sir In majority cases sucrose is used in tissue homogenate preparation as it acts as a cushion, and give you better separation of cell fractionation, less contamination between these fraction , also it maintains osmotic strength of the buffer while having low ionic strength. Furthermore, for low ionic strength, sucrose is fine in case of higher ionic strength Mannitol is recommended. Mannitol elevates blood plasma osmolality, resulting in enhanced flow of water from tissues
Dear Mr. Bejawada Chanikya Naidu can you please provide some relevant references.
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