Dear Rakesh,
pCAMBIA has wide range of vector like 1300,2300,2301. it is binary vector kanamycin based with small replicon and has plant selection gene, which can expdrssed in plant system very well.
These are the some suitable criteria for using this vector in transgenic plant development.
Good Luck
The selection of vectors by the research community are principally driven by;
1. Availability and usefulness.
2. pCAMBIA series are high copy number vectors and the selection in plants is Kanamycin or hygromycin gene that is near LB of t-DNA that is in contrast to the earlier widely used vector pBI121 which has kanamycin selection towards RB of T-DNA and are low copy. The low copy hinders in plasmid isolation/cloning. The presence of plant selection markers towards LB helps in eliminating false positive transgenic plants (plants that will be negative for the gene of interest and positive for the plant selection marker) as the T-DNA transfer is from RB of T-DNA from Agrobacterium.
3. Now days many options are there so you see more wide-range of vector series.
1. pCambia vectors are improved version of binary vectors for plant transformation. As mentioned in their website "Descriptions of pCambia vectors", the old binary vectors tend to have the following drawbacks:
(1) low-copy origin of replication resulting in low yield DNA preps
(2) unstable replicons, causing variable loss of the plasmid during propagation
(3) large size
(4) lack of convenient restriction sites for manipulation
(5) limited choice of selectable markers for both bacteria and plants
(6) lack of simple ways to construct reporter gene fusions
2. pCambia vectors are improved to have high copy number in E.coli for high DNA yields for easy cloning
3. pCambia vectors are small size, with adequate Multiple Cloning Site (MCS) for inserting your gene-of-interest
4. pCambia vectors have the most popular promoter: 35S promoter, have the most used plant selectable markers: kanamycin or hygromycin B and have the most used screenable marker: GUS
5. An array of pCambia vectors have been developed which suit different researchers' need for different projects
6. Most importantly, pCambia vectors are an "open source", build on "free-to-operate", everybody can use [see http://www.cambia.org/daisy/cambia/585 for detail]
Thanks for your valuable answers Dr.Yuan-Yeu Yau, Mr.Rama Kant Dubey, Dr.Kamal Kumar.
pCAMBIA vectors are very suitable for cloning with the addition, of the kanamycin selection gene marker, to have the coding sequences for GFP and GUS as reporter genes, and also those reporters are fused (GFP::GUS or GUS::GFP) to follow your gene product with confocal microscopy or selecting the tissues were the gene product is present by X-Gluc degradation.
Regards.
pCAMBIA vector is suitable vector for transformation because high copy number, small size having kanamycin selection gene marker.
The information provided are indeed useful. I have been informed and enlightened. Thank you all.