I am trying to extract DNA from different plant samples. From tomato and tobacco seeds my RNA was remover by CTAB method after grinding with liquid nitrogen. But from green gram seed i am facing problem of RNA contamination i have used 50 -300 microgram per microliter RNase A incubation at different temperature (37-60 degree.for 15 min, 30 min and one hour. i didnt get rid of RNA.
Is it possible beta-mercaptoethanol inhibiting RNase.
i used RNase A from invitrogen, sigma and genei.
can anybody help me in this problem.
Yes, it is very possible that 2-ME in lysis buffer could inhibit RNAse. What you could do to add some RNAse A into your final eluted DNA (Not into lysis buffer). The RNA should be broken down. Be careful though, because from my experience, addition of RNAse A into lysis buffer could result in poor quality DNA.
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