Why do I get a sheared pattern of amplicons in PCR?

More Subramani Pandian's questions See All

For the below attached image, I wish to know the tools available to draw models like that for research paper?

I'm currently working on the cognitive radio network technology. For illustrative purpose I wish to know the tools available for depicting my idea effectively.

23 July 2023 8,112 4 View

Has anyone attempted to transfect ES cells with Cas9 RNP/sgRNA/ssDNA using Lipofectamine?

I am attempting to insert a reporter gene into human embryonic stem cells using Lipofectamine-based reagents such as Lipofectamine 3000, CRISPRMAX, and RNAiMAX. Has anyone had success with these...

25 March 2023 4,119 0 View

NEB Calculation Using Quantum Espresso?

Hello Eveyone, I am new to NEB(Nudged Elastic Band) calculation.Can anyone help me to understand and what is needed to do NEB calculation for MoS2.

08 January 2022 3,466 0 View

What is the viscosity index of edible oil ?

viscosity index available for lubricant oil explaining the quality of lubricant oil . Likewise any possibilities for viscosity index of edible oil relate quality of edible oil

10 December 2021 815 1 View

What is the unit of U3 in Abaqus in the result. degree or radian?

I performed quasi-static analysis on gears in ABAQUS. After analysis, I extracted the U3 parameter to measure the gear rotation. What is the unit deg or rad? I am confused. Similarly, when I...

12 September 2021 7,526 2 View

How to create a predefined stress field from an .odb file in abaqus?

I am working on reseach of residual stress formation in composite materials. I had created a simulation that analyses the stress creation because of heating during assembly. Now i would like to...

19 August 2021 4,877 1 View

Why the white crystals formed in thin film hydration while synthesising nanoliposome?

I'm working in nanoliposome for my Ph.D research in which first i used PC to CH 2:1(W/W) and 5:1ratio using chloroform/ethanol 2:1 ratio in both cases,i did not get the clear film, there was a...

31 July 2021 8,536 2 View

How to proceed Quantum Espresso calculation in a correct way?

Hello Everyone, I am a very beginner to Quantum Espresso. I create input file using Cif2QE (Qetoolkit) for 2H MoS2 monolayer supercell (221). First, I have done ‘scf’ calculation to converge the...

13 June 2021 2,233 13 View

How to Choose Cutoff Energy and k sampling points in unit cell and supercell in CASTEP Academic Version?

Hello Everyone, I am a beginner to DFT calculation using CASTEP Academic Version. I don't know how to choose cutoff energy and k points to unit cell, 221...

27 May 2021 8,004 1 View

Are there someone working on HPRT mutagenesis assay? How to treat cells with HAT media? one time treatment or everyday and incubate for 3 days?

I am very confused about whether to add HAT media to cells once and incubate for 3 to 4 days or to add HAT media to cells every day for 3 to 4 days?

03 August 2020 5,690 0 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

AutoGrid and Autodock 4.2.6 window opens for 1 second then closes and there is no output files?

Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...

28 July 2024 2,136 4 View

Christian Q. Scheckhuber

Hello Subramani,

did you use a DNA ladder on your gel? If yes, were the ladder bands focused? Another possible problem could be the DNA polymerase you used. If possible try a new aliquot.

Cheers, Christian

Subramani Pandian

sir, i have used 1kb ladder, that will gives neat banding pattern, and i have used different taq also......

Klaus Eimert

Hi Subramani,

is this a common problem for all your PCRs/gels or does it only occur when conducting RAPDs/gels?

Regards, Klaus

it will occur only on RAPDs only...