whenever i did PCR for RAPD primers i gets banding pattern like this what will be a reason for this?
Hello Subramani,
did you use a DNA ladder on your gel? If yes, were the ladder bands focused? Another possible problem could be the DNA polymerase you used. If possible try a new aliquot.
Cheers, Christian
sir, i have used 1kb ladder, that will gives neat banding pattern, and i have used different taq also......
Hi Subramani,
is this a common problem for all your PCRs/gels or does it only occur when conducting RAPDs/gels?
Regards, Klaus
it will occur only on RAPDs only...
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