Ezgi Nur Çil

3 Questions 5 Answers 0 Followers

Questions related from Ezgi Nur Çil

Why do 3t3-l1 cells peel off without differentiation?

Hello, When preadipocyte cells were seeded to differentiate and confluent 3 days later, detachment and peeling occurred in all wells when changing the culture medium. 60,000 for 12 wells. I am...

24 July 2023 5,932 3 View

Can the transcription factor activity assay method be used instead of western for PPAR and CEBP?

I m investigating sirtuin1 and sirtuin 2 gene expression in the 3T3-L1 preadipocyte cell line. What would you recommend for protein analysis instead of western? thank you,

02 January 2022 7,855 3 View

What do you think about measuring activity with Sirtuin activity assay, which is a fluorometric method, instead of western blotting?

I m investigating sirtuin1 and sirtuin 2 gene expression in the 3T3-L1 preadipocyte cell line. What would you recommend for protein analysis instead of western? thank you, Ezgi

02 January 2022 568 1 View