Why DNA Polymerase enzyme doesn't solidify at -20 degree celsius temperature ?

More Binoy Kurian's questions See All

What are the samples that shows positive confirmation for ninhydrin test?

Is we have any raw sample extract that show positive confirmation in ninhydrin test?

01 February 2019 7,919 5 View

What are the applications of thermodynamics in biological sciences? Is it possible to connect with some natural metabolic process with examples ?

What are the application of thermodynamics (Physical Chemistry) in Biotechnology ?

07 August 2018 3,328 9 View

What were the job opportunities one should get after acheiving a PhD Degree in Biotechnology both private and government presently in india?

Job opportunities after perceiving a Doctorate degree in biotechnology in India presently?

02 March 2018 931 3 View

Generally in what problematic situations in plants does a molecular phylogeny study should be proceeded?

What were the general inferences concluded after the phylogeny reconstruction?

10 November 2017 7,978 2 View

Is it possible to select a phylogenetic tree by finding the bootstrap value in mega software?

Bootstrap value X mega software

09 October 2017 8,550 2 View

Which phylogeny software will give a single tree with 2 or 3 different combination of plastid gene sequences?

Say for example a combination of MATK and RBCL gene in plants ?

09 October 2017 1,566 3 View

Which software can align both forward and reverse DNA sequences(Direct PCR Product Sequences) and fill gaps for phylogenetic analysis?

How can we get a single sequence from two separate forward and reverse sequenced PCR product ? Is it possible by bioedit software?

08 September 2017 5,862 2 View

What were the basic steps and softwares used in a phylogenetic analysis ?

Can anyone explain it with an example and steps followed?

08 September 2017 1,735 4 View

How horizontal gene transfer between tree species in dioecious plant occurs naturally ?

How does pollen-pistil incompatibility and geographic barriers between plant species in a family favors / disfavor horizontal gene transfer ?

06 July 2017 9,232 3 View

What are the elements in a particular plant of different species from different phytogeography found in different type of sexual systems?

Why transition from moneocious to Dioecious and different forms of Dioecious were found in some plant families? Is this an evolutionary adaptation or due to some other reasons?

06 July 2017 2,006 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View