What are the samples that shows positive confirmation for ninhydrin test?

Is we have any raw sample extract that show positive confirmation in ninhydrin test?

01 February 2019 7,705 5 View

What are the applications of thermodynamics in biological sciences? Is it possible to connect with some natural metabolic process with examples ?

What are the application of thermodynamics (Physical Chemistry) in Biotechnology ?

07 August 2018 2,941 9 View

What were the job opportunities one should get after acheiving a PhD Degree in Biotechnology both private and government presently in india?

Job opportunities after perceiving a Doctorate degree in biotechnology in India presently?

02 March 2018 699 3 View

Generally in what problematic situations in plants does a molecular phylogeny study should be proceeded?

What were the general inferences concluded after the phylogeny reconstruction?

10 November 2017 7,661 2 View

Is it possible to select a phylogenetic tree by finding the bootstrap value in mega software?

Bootstrap value X mega software

09 October 2017 8,310 2 View

Which phylogeny software will give a single tree with 2 or 3 different combination of plastid gene sequences?

Say for example a combination of MATK and RBCL gene in plants ?

09 October 2017 1,363 3 View

Which software can align both forward and reverse DNA sequences(Direct PCR Product Sequences) and fill gaps for phylogenetic analysis?

How can we get a single sequence from two separate forward and reverse sequenced PCR product ? Is it possible by bioedit software?

08 September 2017 5,568 2 View

What were the basic steps and softwares used in a phylogenetic analysis ?

Can anyone explain it with an example and steps followed?

08 September 2017 1,503 4 View

How horizontal gene transfer between tree species in dioecious plant occurs naturally ?

06 July 2017 9,023 3 View

What are the elements in a particular plant of different species from different phytogeography found in different type of sexual systems?

06 July 2017 1,787 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View