How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Neural stem cell clumping during cell expansion? differentiation?

Hello, I am currently working towards neuronal differentiation through the use of rNSCs. I keep running into the same problem, cell clumping. The controls (complete media) clump further than the...

04 January 2021 7,756 5 View

Are these IPScs Differentiated ?

Hi everyone, So these are images of IPSCs 2 days post passaging/splitting. I noticed that the overall morphology of colonies are not compact or round and without distinct border. However, at...

28 December 2020 3,503 3 View

Does anyone else know an alternative to the cytocentrifugation to analyse and stain BAL cells?

Our group is starting to work with BAL samples from mice. One of our aims is to analyze BAL cells by differential cell count. Many authors report doing a cytocentrifugation, but we don't have this...

21 October 2020 2,897 1 View

Numerically investigating whether a function is differentiable?

Hello all, I am looking for an method / algorithm/ or logic which can help to figure out numerically whether the function is differentiable at a given point. To give a more clear perspective,...

11 September 2020 6,072 14 View

What is the optimal well surface area for C2C12 myoblast differentiation into myotubes?

Does anyone know of studies investigating the optimal well sizes for growth/hypertrophy of C2C12 myoblast-derived myotubes following differentiation using standard serum starvation? I find that...

06 July 2020 598 2 View

HT-29 cell line differentiation using Methotrexate (MTX)?

Hello, I am trying to differentiate HT-29 cell line using Methotrexate (MTX). I came across an old article that suggested treating cells for 3 months in order to reach their confluence state,...

26 April 2020 4,964 1 View

Problems faced while stem cell differentiated into NPCs?

07 March 2020 5,689 2 View

Would someone be able to provide assistance with alizarin red s/mineralization assay with SaOS-2 cells?

I'm currently trying to induce and identify mineralization in my SaOS-2 cells. The issue I'm having is that the whole well will stain red, and of course this intensity depends on how long the...

26 January 2020 6,289 5 View