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Questions related from Naveen Kumar Mekala
Hello, Our CRISPR knockout completely altered the sequence of sgRNA target due to which I see seven amino acid (triplet) changes in sequencing. I have sequenced our samples couple of times and...
23 June 2018 3,218 2 View
Hi, I am trying to electroporate human immortalized cardiomyocytes (SV40)and searching for an optimized parameters like voltage, capacitance, time, etc. If someone have experiences in this regard?...
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As we all know RBC lacks mitochondria. There are few explanations like RBC respire anaerobically so it does not require mitochondria, Since RBC does not have genome and it may not support...
23 October 2017 7,144 7 View
I am trying to isolate mono nuclear cells (MNCs) from cord blood (which used to be my routine job during PhD) but I am not able to get the MNC fractions (buffycoat) now. One of my observations is...
23 January 2015 869 6 View
I am using DMEM-LG (low glucose) for osteogenic differentiation of stem cells. We know if we use DMEM with high glucose, stem cells are going to convert in to chondrocytes, but what is the exact...
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