Hello friends,
We treated NHDF cells with different concentrations of graphen to investigate toxicity. After 24, 48, and 72 hours, we collected cell pellets.
Even if we dissolve graphene well, over time graphene collapses and accumulates in the pellet.
My question is, why did the graphene spread over the cells?
Tuba Öz
Graphene does not dissolve, but disperses in water. An unstable dispersed system of water with hydrophobic graphene is formed. NHDF cells are a complex mixture of proteins and other molecules that are united by weak intermolecular interactions, including hydrophobic ones. Such forces hold graphene inside the cell. You can disperse graphene in water, then add soap. Graphene will rise from the bottom of the test tube and disappear into soap micelles, which are formed due to hydrophobic interactions. This process is similar to the distribution of graphene in cells.
Tuba Öz
Disperse graphene in water as you did. Add sodium dodecyl sulfate to this water in such quantity that its concentration in water is 0.015 mol/l. Stir the dispersion system. Graphene will disappear from the bottom of the test tube. You can do it with any soap or shampoo, but I can't tell you the exact concentration. Don't you feel sorry for the graphene? You're rich.
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