How to detect phosphorylated protein by western blot ?

Dear expertise, 1) I have some trouble to get reproducible results of phosphorylated protein from western blot. Please give me some ideas and suggestions to get the perfect/reproducible results...

15 March 2020 6,690 4 View

Could anyone help me with TEM analysis of my bacteriophages?

26 January 2015 9,759 17 View

Could anyone suggest me a method for purifying phages particles in the SM buffer for TEM analysis ?

I performed TEM analysis for my bacteriohage.repetitively I got only salts images how to avoid the presence of salts during TEM analysis.

24 December 2014 6,470 6 View

Why do my Vibrio species change their nature of yellow color to green color on TCBS plates after 36 hour incubation?

22 May 2014 6,754 8 View

Why did I get a small amount of plasmid DNA during bacterial Genomics DNA extraction/ isolation?

see above

09 March 2014 2,327 6 View

Can someone help with computer-aided drug-design platform using PyMOL?

How to The file Settings_Linux.txt needs to be downloaded and copied to your home directory? reff site: http://people.pharmacy.purdue.edu/~mlill/software/pymol_plugins/install.shtml I have...

08 January 2014 2,149 3 View

How to extract and purify the Bacteriophage particles against pathogenic bacteria? - please suggest me good ideas and research articles

Need suggestion, good ideas and research articles

17 June 2013 6,974 0 View

Could anyone tell me how to calculate the adsorption rate of bacteria and bacteriophage? with any time duration and/or mathematical calculation

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12 June 2013 1,004 3 View

How to prepare adherence cell line from THP-1 suspension cell line ?

Dear experts, I would like to work on THP-1 as adherent cells without changing its natural. Is there any method or protocol for develop adherence cell from suspension cell lines?

01 January 1970 9,871 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

How we can correct the slide?

i am doing research on E.feacalis that is grown in dentin and after that, we did a gram stain for the sample my Q first does the gram stain consider as reliable tool for testing and seeing the...

14 February 2021 6,697 1 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View