How to check the pH of E. coli culture initially? and how to change the pH of the E. coli culture?

I am working on E. coli 15597 prepared in Luria broth. Initially, the pH of LB is 7 after the inoculation of the E. coli it changes to 8.26 is it correct? can bacteria be alkaline?

15 October 2020 2,042 2 View

What are the best pipleline available for the RNA-Seq data analysis ?

What is the latest pipeline to run the RNA-Seq genomic data ?

12 October 2020 7,618 2 View

If an article is unknowingly published in a fake journal, is it considered as published or can the article be sent to some other journal to publish?

How do we handle this menace of fake journals?

14 July 2020 4,835 7 View

How do we differentiate conservation agriculture and regenerative agriculture in context of sustainable farming practice?

Sustainable farming practices appears into different names confusing the global audience which to follow and why? What makes conservation agriculture as different approach than regenerative...

30 June 2020 7,726 2 View

How to get DOI for my published article in a journal which has not given any DOI?

I want to know if Research Gate generates DOI for articles which has been published but has not been provided any DOI by the publisher?

23 May 2020 510 7 View

How many times do we have to check and report model fit measures during SEM?

I am trying to run a SEM which has four latent variables in it. Let us consider these variables as A, B, C and D. All are latent. Here A and B are independent variables, C is mediator and E is...

17 May 2020 4,091 3 View

What is the criteria to calculat e the plagarism?

What is the criteria to calculat plagarism for writing manuscript

06 April 2020 6,936 3 View

What happens if we lower the number of cycles in explicit dynamics for ansys ?

Hi I am a student pursing my final year in mechanical engineering. I am doing crash analysis of the car frame in Ansys software in explicit dynamics module. I had my analysis running for 2-3 days...

18 March 2020 3,709 3 View

Can authentication in IoT be implemented using BlockChain techniques?

Authentication in IoT systems are vastly insecure. Mostly the problems lies on its open ended and probable untrustworthy nodes. Also, the need for a lighter authentication mechanism will always be...

09 December 2019 4,896 4 View

How can I calculate excited state raman intensities in Gaussian09 ?

I am doing calculation to see excited state and ground state raman intensities. I have successfully done it for ground state using dft and after finding absorption wavelength , have optimized the...

08 November 2019 7,920 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View