As the question suggests, I would like to know why glycerol can't be used on DAB stained slices.
There's also something that's always been bugging me. I used a Roche TUNEL staining kit earlier and the manual specifically said that glycerol can be used to seal the cover slips before looking at the results on a microscope, but the end product was stained by DAB. So was the manual wrong to say that, or is it just not something to be concerned about?
I would like to thank anybody who looks and answers this question beforehand; this is a great place to get answers.