8 Questions 24 Answers 0 Followers
Questions related from Yun Li
As stated in the question, we want to try out this new method for calcium via flow cytometry but not sure if the dye would really work or not. Anyone has experience with this sort of thing?
11 July 2019 5,906 0 View
This problem has been persisting for some time, and we've tried everything from perfusing at a slower speed to making sure the 4% PFA solutions are good , we are running out of ideas. This is a...
23 August 2018 287 14 View
So adding mounting medium and then placing the coverslip onto the slide sounds and looks easy enough, but there's still so many things that could go wrong. For one, I just can't seem to be able to...
03 August 2018 1,603 5 View
My lab recently purchased a monoclonal antibody of the peptide Galanin from Sigma-Aldrich. According to the user's manual, the band should be found at about 38kD. The resultant Western blot...
12 December 2015 1,898 8 View
I'm trying out a new neuroprotective substance on animal models of ischemic stroke, so far I could prove that this particular thing could cause caspase 3 activity to decrease at 24h of...
06 November 2014 7,838 6 View
As the question suggests, I would like to know why glycerol can't be used on DAB stained slices. There's also something that's always been bugging me. I used a Roche TUNEL staining kit earlier and...
22 October 2014 7,809 2 View
I would like to see the localization of a specific protein on the peri-infarct region of mice brains that went through ischemia/reperfusion. But it's pretty hard to see the infarction area during...
22 June 2014 2,332 4 View
I'm trying to work through the protocols for TUNEL staining using In situ Cell Death Detection Kit (Roche), and the recipe of the permeabilization solution keeps bugging me. I admit, we never used...
18 March 2014 6,722 8 View
