Various schools have emerged in the study of comparative literature. Mention the most prominent of them in a nutshell.?

Various schools have emerged in the study of comparative literature. Mention the most prominent of them in a nutshell. And he talked about one of them?

09 February 2021 1,099 8 View

How to obtain creep parameters for running Norton Bailey model, material SS-304 ?

How to obtain creep parameters for running Norton Bailey model, material SS-304 ?

06 January 2021 8,074 2 View

How to Interpret Photoluminescence Spectrum of Mixed Pb-Sn FA0.7MA0.3Pb0.7Sn0.3I3 Perovskites?

Dear Researchers, I hope you all will be in good health. I am writing this to request for your guidance. I am working on the fabrication of low bandgap Pb-Sn mixed perovskite with exact...

23 December 2020 1,147 1 View

For designing domain ontology, which methodology should be used?

Is it better to use a predefined methodology? or design own methodology according to the domain, size of ontology, number of participants, the technical level of participants, deadline of the...

14 November 2020 7,972 4 View

How to indicates the most limiting factor /s in different areas within one country ?

I'm creating SDM for an endemic species to a country. The country have different areas where climate, elevation and LULC differ. Is there a tool / way to indicates the most limiting factor /...

12 November 2020 6,161 1 View

Is it feasible to store transactional data in ontologies?

It is not feasible to store transactional data in ontologies. when the size of data will grow, the number of triples in the ontology will be in million or trillion. Due to the un-structured file...

03 November 2020 8,788 3 View

Modeling of entities (endurants) is enough. Why do researchers need to model perdurants in domain ontologies?

Modeling of entities (endurants) is enough. Why do researchers need to model perdurants in domain ontologies? I guess, there is no difference between the following two way of modeling domain...

30 October 2020 5,329 3 View

Which methodology is suitable for designing domain ontology for the Waste Management Domain?

I am designing a domain ontology for the Waste Management Domain, Can anybody suggest a mature, well-documented, and widely accepted methodology for ontology designing and development? Thanks

20 October 2020 2,991 20 View

What is the tool in ArcGIS to generate habitat suitability map using future distribution data of a species?

I have future distribution dataset of a species (from Maxent). I want to create a habitat suitability map using ArcMap.

13 October 2020 2,696 6 View

What are the possible reasons for getting bands for both methylation and unmethylation primer set in MS PCR?

I am currently doing methylation specific PCR and I find that all my samples are showing bands for both methylation and unmethylation in both the control and tumor samples. I wonder is it because...

22 September 2020 6,002 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View