Why, as I am optimizing Desmin staining of Neonatal Rat cardiomyocytes, I am getting weak staining with my Sigma Desmin Antibody?

More Ketaki Mhatre's questions See All

Howto convert solar intensity in LUX to watt per meter square for sunlight?

I am trying to develop a solar dryer. for calculation of efficiency i want to know the incident energy on the dryer. I have a luxmeter in which i can record the sunlight intensity in LUX. But i...

20 December 2018 4,209 15 View

Can AAV or Lentivirus vectors be used for tissue specific expression or knockdown of genes?

I do not have any experience working with LV and AAV's, but planning to use it for kd and overexpression of gene. I was wondering if LV and AAV's can be used as a quick alternative for Cre-ER for...

24 February 2015 6,890 6 View

Can someone suggest some literature on the impact of urbanization on climate change?

How migration, population growth is an additional stress on urban vulnerability.

19 November 2013 9,159 4 View

Will preconditioning cultured isolated adult rat cardiomyocytes with serum help their viability?

I want to culture Adult Rat Ventricular Cardiomyocytes upto 48 hrs. The problem is cell viability which falls rapidly after first 6 hrs of Isolation. My aim is to induce hypertrophy in this...

01 January 1970 4,071 8 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

Ketaki Mhatre

Hello!

I isolate cardiomyocytes and treat them , then fix them Acetone:Methanol for 20 mins -20C and permeabilize the cells with Triton. I block by PBS with 5% BSA for 45 mins then use Sigma Mouse Desmin primary Antibody (IgG)1:50 concentration and Anti Mouse IgG Alexa 488 secondary antibody 1:1000 for staining.

Sandeep Rajput

Hi Ketaki,

May be weak staining indicating the low expression of Desmin after your treatment of cardiomyocytes.

Also sometimes methanol due to auto florescence subside antibody signal. You can increase concentration of primary antibody or try antibody from different company. Best of Luck.