Ketaki Mhatre

2 Questions 5 Answers 0 Followers

Questions related from Ketaki Mhatre

Why, as I am optimizing Desmin staining of Neonatal Rat cardiomyocytes, I am getting weak staining with my Sigma Desmin Antibody?

So, I fix the cells with Acetone:Methanol and permeabilize the cells before staining. Please do suggest where should I start optimizing my protocol. Thanks!

07 June 2015 5,548 2 View

Will preconditioning cultured isolated adult rat cardiomyocytes with serum help their viability?

I want to culture Adult Rat Ventricular Cardiomyocytes upto 48 hrs. The problem is cell viability which falls rapidly after first 6 hrs of Isolation. My aim is to induce hypertrophy in this...

01 January 1970 4,090 8 View