How to calculate the Total FAME after GC analysis according to LAP PROTOCOL 60958?

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Afroza Begum

The total fatty acid content can be calculated as the sum of all individual fatty acids. The relative abundance of each fatty acid can be calculated by dividing the concentration of each individual fatty acid by the total fatty acid content.

Ketaki Nalawade

Thank you Laxmi and Afroja , but what about internal standard recovery and internal standard addition

Hans David

The calculation and processing protocols are well explained in the following paper:

https://doi.org/10.1007/s12161-021-02203-0

I hope this helps!