I'm culturing Hela cell. when the cells reached P4, I froze the cells. then thawing them two weeks later..but the cells started growing at 50% confluency, 48 hours.
What's wrong I am??
I was preparing a seminar and accidentally stumbled upon an odd situation. When there is a negative relationship between the predictor (Extraversion) and the dependent variable (Depression...
12 June 2023 7,476 13 View
Recently, I published two new spider species in Forests and registered two new species in Zoobank after the paper was published. However, the World Spider Catalog currently contains the...
22 March 2023 3,201 2 View
Hi, everyone. I'm master's degree candidate. I research about T cell engineering. I use retrovirus vector, and engineer the T cell through the transduction using retroviral vector. I did...
04 January 2023 6,339 0 View
I try to degradation of natural rubber latex using 20% low ammonia NR latex. "Degradation of NR was carried out by preparing latex with 20% dry rubber content (DRC). The pH of the latex was...
19 June 2022 367 5 View
Hello! I have a problem in calculating hyperpolarizability using Gaussian16. I put the gif file and input the command -> g16 filename.gif filename.out & Then, an error message pops up and...
24 November 2021 5,403 2 View
Hello I'm new user of TCAD sentaurus. I tried to simulate MIS HEMT using TiO2, but TiO2 was not on the material list. But someone said sentaurus can use TiO2 as high-k materal. How can I use it?...
14 September 2021 4,723 2 View
The possibility of semiconducting BaSi2 films for potential application in thin-film solar cells has already been explored by several research groups. Literature review reveals that to grow...
18 May 2021 5,775 5 View
How do you adjust the base layer thickness of agarose gel when electrophoresis in a comet assay? When I was doing peparation of Slides, dip conventional slides and wipe underside of slide and...
28 December 2020 6,758 2 View
In materials science, a dislocation (line defect) represents a linear crystallographic defect within the crystal structure, which causes an abrupt change in the arrangement of atoms, which in turn...
27 July 2020 4,495 7 View
I am designing a Neural Network with two hidden layers for a regression problem in Python. While all inputs are positive, there are supposed to be negative values in the output. The model was...
09 July 2020 6,490 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
There is several approaches towards the analysis of legal status of people with disabilities (e.g. medical, social or human rights approach). One way to include people with disabilities in society...
17 July 2024 3,798 13 View
What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...
13 July 2024 2,431 2 View
I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...
08 July 2024 9,201 2 View
I’m currently reprogramming my fibroblast cells using Simplicon RNA OKSG-cMyc TagRFP Kit and after 13 days, these colony/aggregates started to show up in all of my wells. I have 6 wells, 2 wells...
07 July 2024 9,034 2 View
Usually, basal media have been recommended for the culturing of human epidermal keratinocytes, and most articles suggest growing them in serum-free media. I'm finding it a bit challenging to...
24 June 2024 2,609 3 View
My liposome is a non-phospholipid liposome (sterosome) composed of palmitic acid and cholesterol, 20mg of palmitic acid and 70mg of cholesterol. After several months of storage in a refrigerator...
18 June 2024 7,459 5 View
I have been trying to culture P. falciparum, but I see contamination after ~1 week of culturing. What could this organism possibly be (image attached)? The culture media consists of RPMI, HEPES,...
15 June 2024 5,515 5 View
Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...
10 June 2024 4,152 1 View