Hi, everyone.

I'm master's degree candidate.

I research about T cell engineering.

I use retrovirus vector, and engineer the T cell through the transduction using retroviral vector.

I did the transfection to Plat E cell to collect virus particle.

After 48h later, Harvest the soup and treat to T cell and spin transduction. (Retronectin coated plate, 2000rpm, 90min)

After spin transduction, 48h later, I changed the media and check the transduction efficiency. (It is lab's protocol}

The retroviral vector has IRES GFP, so I checked the expression of GFP through the flow cytometry, but it is low.

How can I increase the efficiency of that?

Thanks