I noticed it already some time ago, that in accordance to suppliers MSDS some enzymes have actually higher activity in other-than-supplied buffer. Why do they not provide the best buffer?
If you go to http://www.clontech.com/takara/US/Products/Molecular_Biology/Restriction_Enzymes/Restriction_Enzymes_g_h/HindIII and click on Documents and load the data sheet, there is a table of relative activities. HindIII should have 100% activity in M buffer (supplied), but 200% in K buffer. So why not use K buffer instead of M?
The suboptimal buffer which supplied with will greatly improve ease of endonuclease use, especially when carrying out in double-digested system!
Yes, it is to facilitate double digestions with non-compatible enzymes.
but similarly one may need to do the double digestion in other buffer. And yes, I've seen few times that there was star activity in the other buffers, but I picked example where this is not the case.
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