Jorge Andres Sanchez-Perez

5 Questions 3 Answers 0 Followers

Questions related from Jorge Andres Sanchez-Perez

Mycobacterium tuberculosis: Treatment prior Susceptibility Testing?

hello all, A research group and I are finding out the antimicrobial activity of A. fumigatus over susceptible and resistant strains of M. tuberculosis. We are proceeding the MOPS method using the...

24 May 2019 9,710 2 View

How to determine a breaking point of susceptibility by disc diffusion?

I am evaluating an organic extract of Aspergillus on Staphylococcus aureus using a 6 mm disc in agar Muller Hinton. I am want to determine a breaking point, is it enough to say that a halo of...

16 January 2019 1,224 3 View

Have you ever used CHLOROFORM to extract secondary metabolites from fungi?

I used 50 mL of Aspergillus fumigatus liquid fermentation (fermentation in SPG broth for 10 days) plus 30 mL of chloroform in a decantation funnel during 3 days. I got the organic phase and...

01 June 2018 5,766 3 View

How to EXTRACT Aspergillus metabolites using ETHYL ACETATE?

Hello all, According to my research protocol I could filter the liquid fermentation of a clinical isolate of Aspergillus fumigatus which was grown in SPG broth for 14 days in automatic rotation...

17 April 2018 9,155 3 View

Aspergillus fumigatus Metabolites extraction by ethyl acetate. Before o during fermentation step?

I am working with clinical isolates of Aspergillus fumigatus in order to get secondary metabolites in order to prove antimicrobial activity. I will reactivate, do a monosporic culture, and then to...

05 January 2018 2,850 3 View