Hi, I am trying to set Golgi-Cox staining protocol in our lab, using the Super Golgi kit (Bioenno https://bioenno.com/products/supergolgi-kit), for mouse brain coronal slices (200um). I have a serious problem with slices over-dried (even though I worked according to the kit protocol, step by step). The slices totally broke while washing them 4 times at ethanol 100%. Do you have any ideas about which changes I have to try to overcome this problem?

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