Why are DNA FISH probes able to bind in the presence of formamide?

03 April 2019 0 4K Report

It's my understanding that formamide participates in hydrogen bonding with DNA, which is why it is able to keep the two strands separated. This principle should apply to small ssDNA FISH probes (100-200nt), so why are FISH probes still able to bind their target DNA sites in the presence of 30-60% formamide?

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