Behnam Javidfar

9 Questions 6 Answers 0 Followers

Questions related from Behnam Javidfar

Why are DNA FISH probes able to bind in the presence of formamide?

It's my understanding that formamide participates in hydrogen bonding with DNA, which is why it is able to keep the two strands separated. This principle should apply to small ssDNA FISH probes...

03 April 2019 4,330 0 View

What's the maximum temperature at which small RNA (100-200nt) will lose its secondary structure without complete degradation?

I have some RNA transcripts about 130nt long on a Bioanalyzer run, and they should be closer to 100nt. I think it's due to secondary structure. What's the maximum temperature at which I can...

22 March 2019 9,023 1 View

Does DMSO precipitate DNA?

I'm running a conjugation reaction to label 20nt oligonucleotides with an NHS ester fluorophore. The oligos have a 3' amino group to anchor the ester. I dissolved the NHS ester in 100% DMSO and...

13 November 2018 4,104 3 View

How to inject midline thalamic nucleus in mouse adult brain?

I'm trying to target the paraventricular nucleus with an AAV-Cre-GFP and I'm having trouble being able to inject this region. I suspect the 3rd ventricle being located right above it isn't doing...

31 January 2017 5,079 1 View

How many nucleoli per neuron in mouse cortex?

Based on some images, it looks like there can be variability in the exact number, but does anyone know of any literature that describes the diversity of neuronal nucleoli and gives exact numbers...

11 January 2017 1,980 2 View

Anyone know of a protein that could be used to fill neurons during recording for fluorescent labeling?

I can't use biocytin for the imaging protocol I intend to put the tissue sections through after ephys. Is there a protein that binds to neuronal membranes that I can use a conventional secondary...

02 December 2016 2,278 7 View

Is there a way to detect dendrodendritic synapses in mouse brain tissue without using EM?

For example, in olfactory neurons, EM has shown dendritic synapses between Mitral cells and Interneurons. But are there ways to detect such contacts using fluorescence microscopy? Are there any...

22 October 2016 319 2 View

Can post-fixation and sucrose incubation be done together after perfusion?

For mouse brain, can I do post-fixation and 30% sucrose incubation simultaneously and get as good a result as the standard post-fixation followed by sucrose?

25 May 2016 6,150 4 View

Does SDS break down Sulforhodamine 101-hydrazide?

There is a several-day 8% SDS incubation required for mouse brains I'm studying, and I'd like to label the astrocytes with SR101-hydrazide prior to fixing the sections and then placing them in...

17 June 2015 7,663 1 View