I am looking for VEGF A expression in mice placental lysates. However, according to the company (I use primary antibody from Abcam), I was supposed to see band 27kDa, however, I see 37kDa. They have shown 3 bands in mouse cell lines (27, 37 and 50 kDa). However, they say 27 kDa is a VEGF A protein. Could anybody please explain?
Hello Nirupama Ramadas
You are seeing a band 37kDa instead of 27kDa because VEGF-A is glycosylated.
VEGF-A is known as a heavily glycosylated protein. The high molecular weight band (37kDa) that you see is the dimeric form (glycosylated) of the VEGF-A monomer. There is also a trimeric form (50kDa) of VEGF-A which is also heavily glycosylated.
Best.
Hi,
Thank you for the explanation. I highly appreciate it. So I see 2 bands between 35-55 kDa. I believe it could be due to the dimeric and trimeric form of VEGF A.
Sincerely,
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