Why am I getting low concentration of digested plasmid after purification?

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Guanfan Mao

Usually, I digest 2 ug plasmid to creat vector,and digest 4 ug plasmid to creat fragment.What is the "concentration goes down" mean? Appear smear or just weak band? If you use Gel Extraction Kit to purify, I suggest you try low rotational speed to load-column.(~2000/3000rpm)

Christian Praetorius

Can you tell some values (e.g. if you digest 1ug of plasmid, you get only 50ng of purified and digested endproduct)? How do you digest and how do you purify?