When we are estimating the hydrolysable capacity to different organic P compounds by the action of phosphatases, we consistently found that acid phosphatase are more efficient than alkaline phosphatase in hydrolysing relatively resistant organic P compounds like phytin and lecithin but there efficiency is at par with alkaline phosphatase in hydrolysing easily degradable organic P compounds like glycerophosphates. What are the possible reasons for that?
The possible reason could be:
Its more about the enzyme assay system, where use of organic acid base buffer. Eg. Acetate buffer made up of acetic acid, now these organic acids or acidic condition aids in sobilizations of insoluble phytate (eg. phyin), where in alkaline condition it not possible. And soluble phytate is more susceptible for enzymatic action than insoluble one. Find attach relative article showing role of organic acid in mineralizing insoluble phytate.
Dr Patel we are adding phosphatase enzymes to the organic P compound like Phytin or lecithon and estimating hydrolysable inorganic P. So, there is no point the involvement of organic acids here.
Dear Dr. Trafdar, again I am trying to understand, what is the final pH of system where you are adding acid / alkaline phosphate? I am eager to know the detail, Because it more about the solubility of phytin, at acid pH it is more soluble, pic is attached.
Dear Dr. Tarafdar, I assume for both the acid and alkaline phosphatase the respective pH would have been taken to get optimum activity. In fact I would be more interested to know the expirement detail in person (mail: [email protected]).
However, 6.5 is good pH for solubility of phytin, and again soluble form of phyate is more susceptible for action of phosphatase or phytase and release free P. Find the attached article showing the use of enzyme in phytate hydrolysis at different pH. Once phytin hydrolyzed along free P it also generates lower form of inositiol phosphate (depending on type of 3 or 6-phytase), which can be again hydrolyzed by phosphatase.
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