In yeast two hybrid system of protein interaction method 3 AT is added to media of the yeast. What is the main reason behind the using of AT in Y2H?
By applying 3-AT to a yeast cell culture which is dependent upon a plasmid containing HIS3 to produce histidine (i.e. its own HIS3 analogue is not present or nonfunctional), an increased level of HIS3 expression is required in order for the yeast cell to survive. This has proved useful in various two-hybrid system, where a high-affinity binding between two proteins (i.e., higher expression of the HIS3 gene) will allow the yeast cell to survive in media containing higher concentrations of 3-AT. This selection process is performed using selective media, containing no histidine.
Hi there,
Read below:
https://www.researchgate.net/post/How_does_3-amino-1_2_4-triazole_3-AT_works_on_Yeast_two_hybrid_analysis
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