I need the cDNA sequence of protein IE2 of virus HCMV to proceed with a cloning
https://www.uniprot.org/uniprot/P19893
thank you!!!
https://www.ncbi.nlm.nih.gov/nuccore/FJ616285.1
Go to the IE2 CDS and click on it, and then view the result as FASTA:
gene complement(169859..173255) /gene="UL122" /note="beta gene; immediate early gene" CDS complement(join(169859..171342,172888..173072, 173185..173255)) /gene="UL122" /note="interacts with basal transcriptional machinery and cellular transcription factors; specific DNA-binding protein; involved in gene regulation" /codon_start=1 /product="regulatory protein IE2" /protein_id="AAR31449.1" /translation="MESSAKRKMDPDNPDEGPSSKVPRPETPVTKATTFLQTMLRKEV NSQLSLGDPLFPELAEESLKTFERVTEDCNENPEKDVLAELGDILAQAVNHAGIDSSS TGPTLTTHSCSVSSAPLNKPTPTSVAVTNTPLPGASATPELSPRKKPRKTTRPFKVII KPPVPPAPIMLPLIKQEDIKPEPDFTIQYRNKIIDTAGCIVISDSEEEQGEEVETRGA TASSPSTGSGTPRVTSPTHPLSQMNHPPLPDPLGRPDEDSSSSSSSCSSASDSESESE EMKCSSGGGASVTSSHHGRGGFGGAASSSLLSCGHQSSGGASTGPRKKKSKRISELDN EKVRNIMKDKNTPFCTPNVQTRRGRVKIDEVSRMFRNTNRSLEYKNLPFTIPSMHQVL DEAIKACKTMQVNNKGIQIIYTRNHEVKSEVDAVRCRLGTMCNLALSTPFLMEHTMPV THPPEVAQRTADACNEGVKAAWSLKELHTHQLCPRSSDYRNMIIHAATPVDLLGALNL CLPLMQKFPKQVMVRIFSTNQGGFMLPIYETAAKAYAVGQFEQPTETPPEDLDTLSLA IEAAIQDLRNKSQ"
Human herpesvirus 5 strain Towne, complete genome
GenBank: FJ616285.1
GenBank Graphics
>FJ616285.1:c173255-173185,c173072-172888,c171342-169859 Human herpesvirus 5 strain Towne, complete genome ATGGAGTCCTCTGCCAAGAGAAAGATGGACCCTGATAATCCTGACGAGGGCCCTTCCTCCAAGGTGCCAC GGCCCGAGACACCCGTGACCAAGGCCACGACGTTCCTGCAGACTATGTTGAGGAAGGAGGTTAACAGTCA GCTGAGTCTGGGAGACCCGCTGTTTCCAGAGTTGGCCGAAGAATCCCTCAAAACTTTTGAACGAGTGACC GAGGATTGCAACGAGAACCCCGAGAAAGATGTCCTGGCAGAACTCGGTGACATCCTCGCCCAGGCTGTCA ATCATGCCGGTATCGATTCCAGTAGCACCGGCCCCACGCTGACAACCCACTCTTGCAGCGTTAGCAGCGC CCCTCTTAACAAGCCGACCCCCACCAGCGTCGCGGTTACTAACACTCCTCTCCCCGGGGCATCCGCTACT CCCGAGCTCAGCCCGCGTAAGAAACCGCGCAAAACCACGCGTCCTTTCAAGGTGATTATTAAACCGCCCG TGCCTCCCGCGCCTATCATGCTGCCCCTCATCAAACAGGAAGACATCAAGCCCGAGCCCGACTTTACCAT CCAGTACCGCAACAAGATTATCGATACCGCCGGCTGTATCGTGATCTCTGATAGCGAGGAAGAACAGGGT GAAGAAGTCGAAACCCGCGGTGCTACCGCGTCTTCCCCTTCCACCGGCAGCGGCACGCCGCGAGTGACCT CTCCCACGCACCCGCTCTCCCAGATGAACCACCCTCCTCTTCCCGATCCCTTGGGCCGGCCCGATGAAGA TAGTTCCTCTTCGTCTTCCTCCTGCAGTTCGGCTTCGGACTCGGAGAGTGAGTCCGAGGAGATGAAATGC AGCAGTGGCGGAGGAGCATCCGTGACCTCGAGCCACCATGGGCGCGGCGGTTTTGGTGGCGCGGCCTCCT CCTCTCTGCTGAGCTGCGGCCATCAGAGCAGCGGCGGGGCGAGCACCGGACCCCGCAAGAAGAAGAGCAA ACGCATCTCCGAGTTGGACAACGAGAAGGTGCGCAATATCATGAAAGATAAGAACACCCCCTTCTGCACA CCCAACGTGCAGACTCGGCGGGGTCGCGTCAAGATTGACGAGGTGAGCCGCATGTTCCGCAACACCAATC GCTCTCTTGAGTACAAGAACCTGCCCTTCACGATTCCCAGTATGCACCAGGTGTTAGATGAGGCCATCAA AGCCTGCAAAACCATGCAGGTGAACAACAAGGGCATCCAGATTATCTACACCCGCAATCATGAGGTGAAG AGTGAGGTGGATGCGGTGCGGTGTCGCCTGGGCACCATGTGCAACCTGGCCCTCTCCACTCCCTTCCTCA TGGAGCACACCATGCCCGTGACACATCCACCCGAAGTGGCGCAGCGCACAGCCGATGCTTGTAACGAAGG CGTCAAGGCCGCGTGGAGCCTCAAAGAATTGCACACCCACCAATTATGCCCCCGTTCCTCCGATTACCGC AACATGATCATCCACGCTGCCACCCCCGTGGACCTGTTGGGCGCTCTCAACCTGTGCCTGCCCCTGATGC AAAAGTTTCCCAAACAGGTCATGGTGCGCATCTTCTCCACCAACCAGGGTGGGTTCATGCTGCCTATCTA CGAGACGGCCGCGAAGGCCTACGCCGTGGGGCAGTTTGAGCAGCCCACCGAGACCCCTCCCGAAGACCTG GACACCCTGAGCCTGGCCATCGAGGCAGCCATCCAGGACCTGAGGAACAAGTCTCAGTAA
That is just the coding region from ATG start to TAA stop codons. The 5' and 3' noncoding regions are not annotated, so you will have to look somewhere else if you need to know the transcription start and end sites.
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