Enrico, I have thought to do this; however, I cannot find out a mouse antibody to do it. Do you know it?

hai helena, you can try out Immunoflurorescence or immunopercipitation for NF-KB p105 in mouse cellines after culture

did you consider using a NF-kB reporter cell line (fluorescence signal) ?

No, Richard. How can I do?

Thank you Sheikh! I will consider it. Can you tell me about your experience with it (which antibody I have to buy, etc)?

Can you tell me a little bit more about the application in which you want to use it ?

The reporter assay which I'm reffering to isn't published in it's current form.

in short: you transfect a reporter gene (luciferase) with a specific promotor into your cell (line) of choice, challenging this cell (line) with for instance a TLR ligand -> TLR receptor -> NF-kb -> pro-inflammatory cytokines. Because of this reporter gene this cell produces a fluorescence signal (for highest NF-kb specificity they tell me qPCR is the method of choice)

Of course, Enrico. I wanna know which antibodies are they using for?

For FACS, I am pretty sure Abcam has it (for human, predicted to work w/mouse.)

I've done P65 from BD in mouse and gotten results using ebioscience intranuclear buffer set.