Best analysis for this longitudinal study?

Hi everyone, I need help for what analysis is better for this longitudinal study. In my study, I measured the attachment security of children at 5 time points. My dubts are that: 1) spacing...

07 June 2020 9,834 2 View

Cardiac rupture following electrocution in birds: is it common?

24 November 2019 9,676 2 View

Could you suggest a microplate reader for bacterial kinetics?

I would like to purchase a microplate reader to assay bacterial growth kinetics when exposed to different conditions (i.e.:salt concentration, different sugars, etc.). Ideally, I am looking for an...

11 September 2019 3,129 2 View

Can anyone share a good protocol for coating plates/dishes/coverslips for mouse hematopoietic stem cells?

I am currently facing problems with my cell attachment. Can anyone help me suggesting a protocol for coating plates/dishes/coverslips with Poly-L-Lysine for hematopoietic stem cells? Would you...

12 November 2018 6,693 6 View

Did someone use an antibody against caspase-9 for immunoprecipitation from mouse cell lysates?

14 December 2015 9,546 2 View

Can anyone suggest some good laboratories in Northern Italy for standard environmental testing?

I need to identify some good Labs placed in Northern Italy to be able to make vibrations, thermal cycling, ingress protection, salt spray and cold soak tests for automotive application (and not...

11 November 2015 5,518 5 View

Does Someone know if FITC is soluble in chloroform?

I know it is soluble in ethanol, acetone, DMSO, DMF; someone knows about chloroform?

19 March 2015 4,740 2 View

Why don't my EBV transformed B cells secrete antibody?

Dear all, I transformed with EBV B cells from total PBMC and after 1 month I isolated IgG+ cells using Miltenyi beads. Keeping in culture these cells with CpG and without, I got proliferation but...

16 March 2015 9,839 5 View

Does anyone know which primers should I use to genotype CSHL gene trap lines?

I am trying to genotype some CSHL gene trap lines in A.thaliana and I am having some difficulties in designing the primers. For those on the flanking regions (ie, the LP and RP), I used the iSct...

12 February 2015 2,011 3 View

Fluorescein stabilization in ethanol solution for temperature measurements

I'm working since several months with a solution of Fluorescein Disodium (C20H10Na2O5) and Ethanol. So far, the spectrum of this solution is changing due to unknown parameters and I would like to...

01 January 1970 7,629 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

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28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View