Best analysis for this longitudinal study?

Hi everyone, I need help for what analysis is better for this longitudinal study. In my study, I measured the attachment security of children at 5 time points. My dubts are that: 1) spacing...

07 June 2020 9,834 2 View

Cardiac rupture following electrocution in birds: is it common?

24 November 2019 9,676 2 View

Could you suggest a microplate reader for bacterial kinetics?

I would like to purchase a microplate reader to assay bacterial growth kinetics when exposed to different conditions (i.e.:salt concentration, different sugars, etc.). Ideally, I am looking for an...

11 September 2019 3,129 2 View

Can anyone share a good protocol for coating plates/dishes/coverslips for mouse hematopoietic stem cells?

I am currently facing problems with my cell attachment. Can anyone help me suggesting a protocol for coating plates/dishes/coverslips with Poly-L-Lysine for hematopoietic stem cells? Would you...

12 November 2018 6,693 6 View

Did someone use an antibody against caspase-9 for immunoprecipitation from mouse cell lysates?

14 December 2015 9,546 2 View

Can anyone suggest some good laboratories in Northern Italy for standard environmental testing?

I need to identify some good Labs placed in Northern Italy to be able to make vibrations, thermal cycling, ingress protection, salt spray and cold soak tests for automotive application (and not...

11 November 2015 5,518 5 View

Who are the worldwide experts/opinion leaders in primary cancer cells 3D culture?

25 October 2015 1,063 3 View

Does Someone know if FITC is soluble in chloroform?

I know it is soluble in ethanol, acetone, DMSO, DMF; someone knows about chloroform?

19 March 2015 4,740 2 View

Why don't my EBV transformed B cells secrete antibody?

Dear all, I transformed with EBV B cells from total PBMC and after 1 month I isolated IgG+ cells using Miltenyi beads. Keeping in culture these cells with CpG and without, I got proliferation but...

16 March 2015 9,839 5 View

Does anyone know which primers should I use to genotype CSHL gene trap lines?

I am trying to genotype some CSHL gene trap lines in A.thaliana and I am having some difficulties in designing the primers. For those on the flanking regions (ie, the LP and RP), I used the iSct...

12 February 2015 2,011 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View