Hello, Do you have experience with this type of melting curve? Can anyone explain to me why there is a lowering of the curve? I'm using SYbr select master mix on quant studio 5 real time pcr.
I'm having a problem with SDS-gel. I cannot get the protein bands to run fully on the gel. For me it's the first time with SDS-gel. I prepared a polyacrylamide gel with 5% stackyng gel and 15%...
10 March 2024 228 2 View
According to enzymatic method for extracting extracellular DNA in biofilm matrix, is suggested to use CTAB solution. Why? I only need extracellular DNA... Do you have a similar protocol to...
20 February 2023 2,297 1 View
Hello everyone! I am looking for papers having as topic/subject what follows: 1. Immigrant adults that learn italian as a second language in Italy 2. Adults who study italian as a foreign...
03 February 2023 8,195 4 View
dear all, I am looking for a bit of advice about solubility of fluorescent lipids in oil (DEP or mineral oil). my protocol to create an oil+lipids mixture says (at least) 5 cycles of heating...
29 September 2022 4,596 0 View
Good evenining, I have panel data with fixed effects and my variable of interest is standardised. My outcome variable is binary (decision to implement a trade policy). My coefficient is: 0.019....
28 August 2022 7,946 1 View
Is there anybody who has interesting articles about Awake Surgery in bilingual/multilingual patients? Maybe some comparisons with monolingual patients undergoing the same procedure. Thank you!
09 January 2022 2,776 7 View
Hello everybody I need a piece of advice on this topic: I am working with oil in water droplets decorated with a fluorescent tag and ssDNA To avoid droplets' coalescence I need to add a...
10 October 2021 6,002 3 View
Hi, I'm looking for some scientific literature regarding the role of SLP in voice changing for transgender patients. Could anybody help me? Thank you!
15 September 2021 2,488 0 View
Hi everyone, I'm looking for some articles about how scoliosis can affect swallowing leading to dysphagia. Can anyone help me? Thank you! Alessia
13 September 2021 326 3 View
Hi, I am having some problems with my PCR. I have performed a RNA extraction with RNeasy Mini Kit, and after that, DNase treatment (RQ1 RNase free Dnase, Promega) on 2ug of the RNA obtained from...
07 June 2021 2,113 4 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....
05 August 2024 9,366 2 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering
05 August 2024 1,660 0 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...
04 August 2024 3,196 2 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View