While Valadating Microarray data with qPCR, do we need to choose the upregulated and downregulated genes randomly ?

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Leonardo Cardoso Alves

I agree with Jochen and also suggest you to choose genes of interest so you maybe can be able to use these data in your discussion besides the array validation.

Cheers

Pedro Nicolau Neto

In addtion, you should also analyze genes that did not showed expression difference as control and could propose new housekeeping for your study.

Sonti Roy

Thanks @ Jochen Wilhelm, Leonardo Cardoso Alves , Pedro Nicolau Neto