Leonardo Cardoso Alves

4 Questions 12 Answers 0 Followers

Questions related from Leonardo Cardoso Alves

Root DNA extraction without Nitrogen freezing or grinding in mortar?

Hi there, We'd like to extract DNA from microbes living in soybean, wheat and sugarcane roots, but we can't use liquid nitrogen by now for grinding some of these hard materials. Also gringing with...

06 October 2019 1,952 3 View

Removing silicate from samples prior to DNA extraction. Any help?

Hi there, I've got these bacterial samples and were to extract DNA for posterior 16S DNA sequencing. I have two groups of samples: group A are isolates with nothing than bacteria cells (I hope =)...

08 April 2019 6,859 5 View

How to set up primers-pair combinations for high-throughput indentification of fungi?

Hey there, I am looking for primer pairs for fungal amplicon sequencing (ITS2 region) using MiSeq. However, what I found a little bit confusing is that the papers refer to degenerate primers such...

21 November 2018 6,350 3 View

Selection of Arabidopsis transgenic plants from CSHL T-DNA lines

Hello fellows, As I am new in the T-DNA area, maybe this is a very silly question; however I am very confused because of that. I have T-DNA lines from SALK and have already sowed them in culture...

12 May 2017 911 10 View