Non motile black dots in animal cell culture, tightly adhered to the cells along the cell periphery. Is it a contamination? How to get rid of them?

I am seeing non-motile black dots of almost equal size along the periphery of skin melanoma cell line A375. They are present in almost all the cells. They don't turn the media turbid. However...

18 January 2021 5,151 1 View

Best flow cytometry markers for Thp1-derived M1 and M2 macrophages?

Hi everybody, I am trying to polarize and differentiate thp1 cells to M1 and M2 macrophages. There are lots of different protocols, so I've been trying different incubation times and...

02 November 2020 8,554 3 View

Have you ever seen two peaks after preparation small RNA library for NGS?

Hi, I have problem with my libraries, I use ClenTag Small RNA Library Preparation Kit by Trilink. The material is RNA from plasma cells. I have already done this two years ago and everythink...

25 August 2020 8,459 1 View

What are the key things to write and publish a paper in a good indexed journal?

The major spices and ingredients to add flavour in a paper for a journal in the domain of Computer Vision and Machine Learning.

27 June 2020 7,967 10 View

How to decide that a work is suitable for a conference or a journal in the field of Computer Science & Engineering?

What are the deciding factors to submit a paper in conference or journal.

21 June 2020 1,604 8 View

Whether Lambert Academic Publishing (LAP) provide printed copies to author? Do authors need paymet or sharing bank setails?

Hello Everyone !! Does anyone have any experience in publishing through Lambert Academic Publishing (LAP)? Recently I got a request mail from them regarding publication. I want to know about the...

25 April 2020 2,651 2 View

How can I write the .rpy and .jnl file in Abaqus cae for windows?

Hello :) I have the Abaqus CAE student version for Windows recently. My problem is: Abaqus does not write the .jnl and .rpy files automatically during a session as I am used to from the Linux...

30 March 2020 2,579 4 View

HSV1 in human tissue - dangerous after the fixation with PFA?

Hi, we are wondering about the HSV1 virus in human tissue. We want to perform in situ sequencing with this tissue, but we are not experienced with the virus-infected samples. Is it possible that...

14 October 2019 8,969 1 View

What details should be followed in MEGA-X for the Multiple Sequence Alignment of 16S data?

I have some 16S RNA sequence with which I want to do the phylogenetic analysis, so Initially I did BLAST against SILVA db and NACBI 16S db and went for MSA in MEGA-X using Muscle and Clustal both...

21 July 2019 9,320 3 View

Can anyone suggest how to incorporate GLYCAM parameters to build a sugar residue's .frcmod file using ambertool I'm missing out bond,angle parameters?

I tried building topology file using leap program but it failed while checking the file and tallying it with my generated . frcmod file isn't working to fill the missing parameters so how can I...

21 March 2019 225 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View