Vidushi, who told you this? Complete clotting is required before centrifugation, but breaking? What for?

break the clot? what do you mean ?

In general and Regardless of the meaning of your question you don't need to do that .

thanks

Serum separator collection tubes contain a gel that separates the clot from the serum in whole blood specimens. During centrifugation, the gel moves to create a barrier between cells and serum, providing access to the serum for various chemistry, serology and other procedures. Serum separator tubes are recommended for most testing, but please check individual specimen requirements for restrictions.

Do not centrifuge immediately after drawing the blood. Do not allow the blood to clot with the tube in a horizontal position. Do not allow the tube to stand more than one hour before centrifuging..

Specimens transported by mail or stored more than four hours should be separated from the clot and placed into a transport tube.

In some cases the clot can get stuck to walls of the tubes. So it can occurs that this fact makes difficult the separation of clot from the serum especially in cases of not adequate timing or speed of centrifugation. In these cases a gently detachment of the clot from walls of the tube by mean of a glass stick could be recommended.

after collection 2 hours store by roomtemp., then over night storage by 2-8°C, next day hard spin 3800g and harvest supernatend with low cellcontamination (recepture for preparaqtion from autologues serum-eyedrops).

After formation, the clot undergoes a process of retraction due to the shortening of the fibres of fibrin. In some cases, especially if glass tubes are used, the clot can adhere tenaciously to the walls of glass thus prejudging the outcome of centrifugation. The gently breaking of the clot before centrifugation prevent this drawback especially when a low speed of centrifugation needed

Thank you for the valuable replies.