Since lentiviruses are known to preferentially integrate into the transcriptionally active sites in the genome, I am wondering if there was a simple and effective strategy to identify clones that have the lentiviral insert integrated into the genome but not on any site with functional consequence. I would really appreciate any helpful suggestions.
Thanks
Hi Deepti, performing intergration site analysis actually will be optimal. However, if the downstream applications on which you will be using the transduced cells is the ability of perhaps the transducer receptor (if that be the case) to hold a ligand molecule, then the intergration site analysis shouldn’t be a worry. In my case, I transduce cancer cells with receptors of interested and I don’t have to do perform the integration site analysis.
should you want to do the assay, you can get “Lenti-X integration site analysis kit“ ( Cat # 631263) from Takara. I hope that helps
Hi ! Considering ideally that one particle of LV will infect one cell, how many integration will occur in the cell? Thank you
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