Deepti Tomar

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Questions related from Deepti Tomar

While genetically engineering cells using lentiviral transduction system, should one mandatorily perform lentiviral integration site analysis?

Since lentiviruses are known to preferentially integrate into the transcriptionally active sites in the genome, I am wondering if there was a simple and effective strategy to identify clones that...

02 January 2023 1,404 2 View

Is pSuper.neo.gfp vector a suitable system for stable microRNA over-expression or knockdown for its functional characterization?

I am planning to use pSuper vector system for over-expressing and knocking down a microRNA in breast cancer cells. Although it was initially designed for the purpose of siRNA expression, I am not...

30 March 2015 4,354 1 View