Hi, I'm currently trying to prepare a pollen grain squash slides of Hibiscus syriacus to observe meiosis chromosome. I've used a traditional squash technique, which use young flower bud fixed in Carnoy's solution and then squash pollen sac with 60% acetic acid, and use cover slip to spread chromosome and use liquid nitrogen to eliminate cover slip. But results are not satisfying enough..
Is there any other squash technique you recommend to observe meiosis chromosome? when the chromosomes are in small size?