Yun-Jae Ahn

4 Questions 3 Answers 0 Followers

Questions related from Yun-Jae Ahn

How can I get clear plant tissue with phloroglucinol staining?

Hi, I am trying to observe lignin through phloroglucinol staining with arabidopsis (petal and sepal). The protocol I am using : 2% phloroglucinol in 100% EtOH , O/N...

01 November 2017 2,004 1 View

Do I have to make longer or shorter primer when the PCR is not working

Hi, I am currently doing some PCR experiments for further procedure, and I found out that the primers I made are not working in some samples. I am going to re-design primers, however, in this...

16 August 2017 4,863 24 View

Gel electrophoresis of riboprobes after in vitro transcription supposed to show single band or multiple bands?

Dear all, I am trying to set-up a RNA in situ hybridization experiment. I am reading several protocols for that, and I found one paper says 'if in vitro transcription is performed well, you will...

24 May 2017 4,206 1 View

What causes smearing in electrophoresis with genomic DNA?

Hi, I'm having serious smearing problem doing electrophoresis. I'm preparing probe for genomic in situ hybridization.(GISH) I extracted plant genomic DNA , and used DIG-Nick Translation Kit(Roche)...

18 March 2015 2,024 15 View