Yun-Jae Ahn

6 Questions 4 Answers 0 Followers

Questions related from Yun-Jae Ahn

How can I get clear plant tissue with phloroglucinol staining?

Hi, I am trying to observe lignin through phloroglucinol staining with arabidopsis (petal and sepal). The protocol I am using : 2% phloroglucinol in 100% EtOH , O/N...

01 November 2017 2,014 1 View

Do I have to make longer or shorter primer when the PCR is not working

Hi, I am currently doing some PCR experiments for further procedure, and I found out that the primers I made are not working in some samples. I am going to re-design primers, however, in this...

16 August 2017 4,873 24 View

Gel electrophoresis of riboprobes after in vitro transcription supposed to show single band or multiple bands?

Dear all, I am trying to set-up a RNA in situ hybridization experiment. I am reading several protocols for that, and I found one paper says 'if in vitro transcription is performed well, you will...

24 May 2017 4,217 1 View

What is the difference between using herring sperm and general genome as a blocking DNA?

Hey, i'm doing GISH(Genomic in situ hybridization) with plant material, and I'm struggling with indistinct signals. Proper ratio seem to be different from species to species.. What I'm wondering...

22 February 2016 7,005 3 View

Which way is the best for pollen grain squash technique?

Hi, I'm currently trying to prepare a pollen grain squash slides of Hibiscus syriacus to observe meiosis chromosome. I've used a traditional squash technique, which use young flower bud fixed in...

25 September 2015 3,742 6 View

What causes smearing in electrophoresis with genomic DNA?

Hi, I'm having serious smearing problem doing electrophoresis. I'm preparing probe for genomic in situ hybridization.(GISH) I extracted plant genomic DNA , and used DIG-Nick Translation Kit(Roche)...

18 March 2015 2,030 15 View