Which vector works best for large scale purification of protein using saccharomyces cerevisiae as an expression system?

11 November 2017 0 2K Report

I have been trying to express an eukaryotic protein in E.coli, but in vain. Their have been no induction using IPTG and the vector was of pet series. So, I have planned to clone it in a saccharomyces vector, using yeast expression system. I have a pESC series vector containing FLAg tag and MYC tag as well. Which do you think might work well for getting soluble protein of better yield?

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